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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human Kit ligand protein (26-214AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
Non-conjugated
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
P21583
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA, WB; Recommended dilution: WB:1:500-1:2000
- 浓度:
>95%,Antigen Affinity Purified
- 靶点:
KITLG
- 抗体英文名:
KITLG Antibody
- 抗体名:
Stem cell factor precursor antibody
- 规格:
100μl/50μl/20μl
| 规格: | 100μl | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μl | 产品价格: | ¥880.0 |
| 规格: | 20μl | 产品价格: | ¥440.0 |
保存缓冲液
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.功能
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验淋巴系细胞有B细胞和 T细胞两个系列,其中 B细胞是抗体形成细胞前体( antibody- forming cellprecursor),在胞膜表面具有以单一特异性抗体作为抗原受体,一旦与抗原结合就直接或借助于 T细胞进行增殖分化,结果成为高速度合成和分泌具有与受体同样特异性抗体的细胞,这些细胞总称为抗体形成细胞。 IgG抗体(免疫球蛋白 G), IgA抗体, IgE抗体(或许 IgD抗体)是在浆细胞内形成的。形成 IgG的浆细胞在脾及淋巴结等末梢淋巴系组织和肠粘膜固有膜和肺间质等全身
and pellet as above. Resuspend in 10 mls of PBS. Put 200 ul (3 mg/ml) of the antibody to an eppendorf tube. Add 0.8 mls of PBS. Pellet 1 ml of the cell suspension and discard the PBS supernatant. Add the antibody solution to the cell pellet
双特异抗体(Bispecific antibody)的简介和制作方法
、双特异抗体简介双特异抗体(Bispecific antibody)是含有两个不同配体结合位点的免疫球蛋白分子。自然状态下不存在双特异性抗体,只能通过特殊方法进行制备。以往双特异抗体的制备方法有化学交联法,杂合F(ab')2 分子法和鼠杂交瘤法等。化学交联法生产双特异抗体的异源性,批与批之间的不稳定性,以及抗体特异性易受某些修饰或不当连接而改变的特性,使得该法生产的双特异抗体不适于体内使用。以巯基交联蛋白酶消化片断F(ab')生产的双特异杂交分子,成分虽较均一,但费时费力,且产量很低。杂交瘤法生产
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