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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
, during time series analyses or when screening transfor-mants for expression of a transformed gene. Under such circumstances, existing tech-niques are overly time consuming and yield more RNA than is necessary. The availability of a rapid RNA mini-prep
Plasmid Mini Purification Protocol
. Allow Buffer QC to move through the QIAGEN-tip by gravity flow.The first 2 ml are sufficient to remove all contaminants in the majority of plasmid DNA preparations.The second 2 ml ensure complete removal of contaminants in all situations
【共享】经验:The RNeasy Mini Kit from Qiagen
The RNeasy Mini Kit from Qiagen is designed for RNA extraction, although the basic format is similar to other Qiagen products designed to purify nucleic acids: ion exchange columns specifically bind RNA which is purified by wash steps
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