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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
Goat Anti-Rabbit IgG (H L) Antibody 实验设备 1. Sterile cell culture hood (i.e., biosafety cabinet) equipped with a stereo microscope 2. Inverted microscope 3. Incubator set at 37°C, 5% CO2 4. Water bath set at 37
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
it to increase recovery of DNA. DTT breaks down S-S bonds of antibodies. (34) For Input tubes, add 200 µl of Elution Buffer and set aside at RT. (35) Add 100 µl of Elution Buffer to each tube
Dual- and Triple-Color Fluorospot
实验原理 ELISPOT has, due to its high sensitivity and simplicity, proven valuable for assessing antigen-specific T-cell responses, both with regard to specificity and magnitude. One limitation of the regular ELISPOT method
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