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文献和实验the beadbound cells with a magnet. Discard the bead-bound cells and use the remaining untouched, enriched cell population for further flow sorting into the DC subpopulation(s) of interest 实验试剂 Magnet: (Dynal® MPC™), MPC-L for 1–5 ml
Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
. Note: It is not necessary to wash the culture surface before adding cells or medium. 3) Plate 9.4 × 106 Mitomycin C-treated or irradiated MEFs in a T-175 flask coated with AF and containing 30 mL of MEF medium. 4) The following day
Buffer P1 50 mM Tris-HCl pH 8.0 10 mM EDTA 100 μg/ml RNaseA The buffer and RNaseA can also be ordered from Qiagen separately (catalog numbers 19051 and 19101). Buffer P2 200 mM NaOH 1% SDS Buffer P3
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