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文献和实验Purification of Endotoxin Free mAbs
1) Endotoxin - you must assume that all standard laboratory glassware will be coated with loads of endotoxin. Wherever possible use sterile, specifically endotoxin-free (clinical grade) disposable plastics. If you need to use glassware or other materials
果蝇细胞的培养实验方法(Culturing Drosophila cells)
注:感谢哈佛大学医学院果蝇RNAi筛选中心的支持和提供本实验方法 I. MEDIUM A. S2, S2C, S2*, S2R+, S3, l(2)mbn, Kc(167), & DL2 cells: Schneider's/ 10% FBS/ PS 450 ml Schneider's medium (Gibco #11720-034) (pour off 50 ml into Falcon to save as serum-free
I. MEDIUM A. S2, S2C, S2*, S2R+, S3, l(2)mbn, Kc(167), & DL2 cells: Schneider's/ 10% FBS/ PS 450 ml Schneider's medium (Gibco #11720-034) (pour off 50 ml into Falcon to save as serum-free) ***[S2R+ and Kc cells
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