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文献和实验Protocol Protein Gel Staining Using Coomassie Blue Protein Stain
Materials Polyacrylamide pre-cast gel SimplyBlue™ Coomassie protein stain, 1x pre-mixed solution (Coomassie blue dye binds to proteins (arginine, the aromatic amino acids, and histidine) allowing protein bands to be visualized
Silver Stain is about 10 to 100 times more sensitive than Coomasie Blue Staining; at least 1ng protein using this protocol. Estimation of protein amouts is not advisable since the staining intensity depends of the developing time and vary
Testing for Mycoplasma by Indirect DNA Stain (Hoechst 33258 stain)
stain solution (Prod. No. H6024 ) Vero cells (Prod. No. 84113001-1v1) Mountant (Autoclave 22.2ml 0.2M citric acid with 27.8ml 0.2M disodium phosphate. Add 50ml glycerol. Filter sterilize and store at 4ºC) (Prod. No. M1289 ) Mycoplasma
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