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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Lung-Specific Expression of Mutant p53 as Mouse Model for Lung Cancer
The p53 tumor suppressor gene is the most commonly mutated gene in cancer (1 ,2) and is mutated in 50% non-small cell lung cancer (NSCLC) and 70% of small cell lung cancer (SCLC) (3) . Mutations in p53 commonly reflect exposures
Proteolysis of p53 Protein by Ubiquitous Calpains
of its own mRNA translation have been proposed. p53 has also been reported to display intrinsic proteolytic activity (5 ,6 ). Importantly, p53 can adopt different tertiary structures. These can be monitored with a panel of monoclonal antibodies
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