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文献和实验Recombinant Collagen Trimers from Insect Cells and Yeast
have been unsuccessful, but active recombinant human prolyl 4-hydroxylase has been produced in insect cells and yeast by coexpression of its α- and β-subunits (7 ,7 ). We have recently shown that recombinant human type III collagen with a stable triple helix
Introduction of human 14-3-3 proteins
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
was found near the termination codon of Hes1 (77% homology)--a basic helix-loop-helix transcriptional repressor that is part of the Notch signaling pathway.To determine whether the Hes1 mRNA is actually a target for miRNA-23, the authors used NT2 cells as a model system. NT
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