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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat Interleukin-18 (Il18)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥3468.0 |
| 规格: | 20μg | 产品价格: | ¥1836.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
Il18Uniprot No.:
P97636别名:
Interferon gamma-inducing factor ;IFN-gamma-inducing factorInterleukin-1 gamma ;IL-1 gamma种属:
Rattus norvegicus (Rat)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
22.3 kDa表达区域:
37-194aa氨基酸序列:
HFGRLHCTTAVIRSINDQVLFVDKRN_P_PVFEDMPDIDRTANESQTRLIIYMYKDSEVRGLAVTLSVKDGRMSTLSCKNKIISFEEMN_P_PENIDDIKSDLIFFQKRVPGHNKMEFESSLYEGHFLACQKEDDAFKLVLKRKDENGDKSVMFTLTNLHQS蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
Cloning of the cDNA for interleukin-18 in PC12 and expression in Escherichia coli.Kim S.-J., Kim C.-S., Song K.-Y., Kim J.-S., Jung K.-S. IL-18 translational inhibition restricts IFN-gamma expression in crescentic glomerulonephritis.Garcia G.E., Xia Y., Ku G., Johnson R.J., Wilson C.B., Feng L.Kidney Int. 64:160-169(2003)Function:
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关专题 &NBS p; Interleukin 18 (interferon-gamma-inducing factor) IL-18 is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other Cell s. IL-18 works by binding
-Class (D200-domain) Cytokine Receptor Activity (19): CSF2RB, CSF3R, IFNAR2, IL10RA, IL10RB, IL12B, IL12RB1, IL12RB2, IL13RA1, IL20RA, IL2RB, IL3RA, IL4R, IL5RA, IL6R, IL6ST, IL7R, IL9R, LIFR. Interleukin Receptors (8): IL12RB1, IL12RB2, IL15RA, IL18
「魔高一尺道高一丈」周挺等 Nature 报道分泌型免疫检查点,伪 IL-18 肿瘤免疫治疗异军突起
. Ring 课题组周挺博士以第一作者在 Nature 上在线发表了题为 IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy 的研究成果。该研究发现肿瘤微环境中产生的 IL-18 结合蛋白(IL-18BP)可能充当「分泌型免疫检查点」限制了 IL-18 免疫治疗的效果;作者通过定向进化的方法筛选得到了一种可维持信号转导能力,但不会被 IL-18BP 抑制的 IL-18(decoy-resistant IL
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