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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Cystathionine beta-lyase metC (metC), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Cystathionine beta-lyase metC (metC), partial品名简称:
Recombinant E.coli metC protein, partial货号:
CSB-RP144774Ba规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
metCUniprot No.:
P06721别名:
Beta-cystathionaseCysteine lyase种属:
Escherichia coli (strain K12)蛋白长度:
Partial来源:
E.coli分子量:
46.8 kDa表达区域:
1-392aa氨基酸序列:
MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKHATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFIEQGDHVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSITMEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDAMIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEWLAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLFSMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
Primarily catalyzes the cleavage of cystathionine to homocysteine, pyruvate and ammonia during methionine biosynthesis文献:
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006)研究领域:
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文献和实验相关专题 大肠杆菌的基因工程 基因工程让一切皆有可能 一、原理 1、 E . coli 表达系统 E . coli 是重要的原核表达体系。在重组 基因转化入E . coli 菌株以后,通过温度的控制,诱导其在宿主菌内表达目的蛋白质,将表达样品进行SDS-PAGE 以检测表达蛋白质。 2、 外源基因的诱导表达 提高外源基因表达 水平的基本手段之一,就是将宿主菌的生长与外源基因的表达分成
; 1000g 离心,15 min ,室温;吸出上清液并保留,用100 μL 2×凝胶电泳加样缓冲液溶解沉淀;取10 μL 上清,加10 μL 2× 凝胶电泳加样缓冲液,与20 μL 重新溶解的沉淀进行SDS-PAGE 。 四、注意事项 (1)不同的大肠杆菌表达载体带有不同的启动子和诱导成分。实验者必须根据特定系统和用途决定相应的实验方案。 (2)表达和检测时,应设置对照组,如转化载体和非诱导细胞。 (3)由于大肠杆菌中表达的重组蛋白
实验原理 1. E. coli 表达系统 E. coli 是重要的原核表达体系。在重组基因转化入E. coli 菌株以后,通过温度的控制,诱导其在宿主菌内表达目的蛋白质,将表达样品进行SDS-PAGE 以检测表达蛋白质。 2. 外源基因的诱导表达 提高外源基因表达水平的基本手段之一,就是将宿主菌的生长与外源基因的表达分成两个阶段,以减轻宿主菌的负荷。常用的有温度诱导和药物诱导。本实验采用异丙基硫代-β-D-半乳糖昔
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