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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Lysobacter Enzymogenes Arg-C (ler), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Lysobacter Enzymogenes Arg-C (ler), partial品名简称:
Recombinant Lysobacter enzymogenes ler protein, partial货号:
CSB-EP188874Ba规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
lerUniprot No.:
O87544别名:
/种属:
Lysobacter enzymogenes蛋白长度:
Partial来源:
E.coli分子量:
52.7 kDa表达区域:
220-463aa氨基酸序列:
GVGDIGSSDYCEKDIVCRVKPSAEFLSASKSVARMVFTPKTGYTGYCSGTLLNNSNSPKRQLFWSAAHCISTQKVANTLQTYWLYDATGCDNDTLSDKAVTLTGGATLLHSHATRDTLLLELKSAPPSGAYYAGWNSSAIATKGTAIEGIHHPSGDLKKYSLGSVTALSSTIDGKKPLTKVAWTTGVTEGGSSGSGLFTISSTSGYQLRGGLYGGTSYCSAPSDPDYYSQLDGVWSSIKTYFSP蛋白标签:
N-terminal GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
Cloning of a Lysobacter enzymogenes gene that encodes an arginyl endopeptidase (endoproteinase Arg-C).Wright D.S., Graham L.D., Jennings P.A.Biochim. Biophys. Acta 1443:369-374(1998)研究领域:
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文献和实验578475733 因最近要做蛋白激酶C在糖尿病方面的实验,求助各位高手有没有蛋白激酶C信号通路的图片,最好带注解,十分感谢。 石依信念 我也想要~~~~ 黑胡桃 [img]data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAskAAAGdCAYAAADgwu6RAAAgAElEQVR4Aey9B7xdxZXmu04
下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
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