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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Beta-1,4-galactosyltransferase 3 (B4GALT3), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Beta-1,4-galactosyltransferase 3 (B4GALT3), partial品名简称:
Recombinant Human B4GALT3 protein, partial货号:
CSB-RP007044h规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
B4GALT3Uniprot No.:
O60512别名:
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3 3 domains:N-acetyllactosamine synthase (EC:2.4.1.90) ;Nal synthaseBeta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
67.2 kDa表达区域:
34-393aa氨基酸序列:
LSALFGRDQGPTFDYSHPRDVYSNLSHLPGAPGGPPAPQGLPYCPERSPLLVGPVSVSFSPVPSLAEIVERNPRVEPGGRYRPAGCEPRSRTAIIVPHRAREHHLRLLLYHLHPFLQRQQLAYGIYVIHQAGNGTFNRAKLLNVGVREALRDEEWDCLFLHDVDLLPENDHNLYVCDPRGPRHVAVAMNKFGYSLPYPQYFGGVSALTPDQYLKMNGFPNEYWGWGGEDDDIATRVRLAGMKISRPPTSVGHYKMVKHRGDKGNEENPHRFDLLVRTQNSWTQDGMNSLTYQLLARELGPLYTNITADIGTDPRGPRAPSGPRYPPGSSQAFRQEMLQRRPPARPGPLSTANHTALRGSH蛋白标签:
N-terminal GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.功能2:
Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.文献:
A family of human beta4-galactosyltransferases. Cloning and expression of two novel UDP-galactose:beta-n-acetylglucosamine beta1, 4-galactosyltransferases, beta4Gal-T2 and beta4Gal-T3.Almeida R., Amado M., David L., Levery S.B., Holmes E.H., Merkx G., van Kessel A.G., Rygaard E., Hassan H., Bennett E., Clausen H.J. Biol. Chem. 272:31979-31991(1997)研究领域:
Metabolism请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验A Fluorescence-Based Assay for Core 1 3Galactosyltransferase (T-Synthase) Activity
for T-synthase activity. T-synthase utilizes the acceptor substrate 4-methylumbelliferone-α-GalNAc (GalNAcα-(4-MU)) and the donor substrate UDP-Gal to synthesize the disaccharide product Galβ1,3GalNAcα-(4-MU) structure. This product is specifically
领域奠基之作:Cell 重磅报道可胞外展示的糖基化修饰 RNA——glycoRNA
唾液酸形式 Neu5Ac 和 Neu5Gc。而当样品被 VC-Sia 或 RNase 处理时,则检测不到,进一步证实 glycoRNA 被唾液酸修饰的。 图片来源:Cell 经典的 N - 多糖合成机制有助于 glycoRNA 的产生 蛋白质上有两类主要的多糖,N - 多糖和 O - 多糖,两者都可以被唾液酸化。 研究人员使用遗传学、药理学和酶学的结合方法进行检测,探索 glycoRNA 结构是否与糖蛋白相关多糖结构相关。 IdID CHO 细胞系不能产生 UDP-Gal 和 UDP
Metabolic Labeling of Glycoproteins with Radioactive Sugars
(see ). Abbreviations used: Fru, fructose; Fuc, fucose; Gal, galactose; GalNAc, N‐acetylgalactosamine; Glc, glucose; GlcA, glucuronic acid; GlcNAc, N‐acetylglucosamine; GlcNH2 , glucosamine; Man, mannose; ManNAc, N‐acetylmannosamine; NeuAc, N‐acetylneuraminic acid
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