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文献和实验DNA-Binding Activity of Hypoxia-Inducible Factors (HIFs)
Electrophoretic mobility shift assay (EMSA) provides a fast and sensitive method for detecting sequence-specific DNA-binding proteins ( see Fig. 1 ). Using an end-labeled DNA oligonucleotide as probe, proteins that specifically bind
Analysis of DNA-Binding Activity in Neuronal Tissue with the Electrophoretic Mobility-Shift Assay
to a specific DNA sequence, usually an enhancer element. The defined DNA sequence is presented as a radiolabeled, synthetic oligonucleotide to a protein extract, which contains DNA-binding proteins. These proteins can then bind to the oligonucleotide. The sample
Analysis of DNA Binding Proteins by Mobility Shift Assay
binding proteins. These include the gel mobility shift assay (1 ,2 ), DNase I footprinting analyses (3 ), and the methylation-interference assay (4 ,5 ). Although each technique can be used to obtain valuable information regarding DNA/protein
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