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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
HSP70 Antibody: Biotin
- 抗体英文名：
HSP70 Antibody: Biotin
- 浓度：
请参考说明书
- 应用范围：
请参考说明书
- 保质期：
请参考说明书
- 库存：
大量
- 供应商：
欣博盛生物科技有限公司
- 保存条件：
-20
- 形态：
液体
- 规格：
200 µg
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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