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文献和实验Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors
as donors to produce cloned animals. Recently, we have developed a novel and effective method using roscovitine to synchronize adult bovine granulosa cells in the G0 /G1 cell cycle stage. The resulting fetal and calf survival after transfer of cloned embryos
Detection of Apoptosis in Ovarian Cells In Vitro and In Vivo Using the Annexin V-Affinity Assay
of apoptosis during mammalian development ( 11 , 12 ). Fig. 1. Confocal scanning laser microscopy analysis of PA-1 ovary teratocarcinoma cells. Apoptosis was induced by treating the cells for 6 h with 50 μM roscovitine, a cyclin-dependent
不够,可选择继续培养再检测,这是该方法的一大优越之处. 研究特殊药物对细胞周期的影响(如roscovitine,一种抗癌药、周期蛋白激酶抑制剂),胰酶消化细胞、收集并铺于含药物的培养基上,37℃ 5%CO2 培养2天;除去之前的培养基换上含10%(v/v)alamarBlueTM 的新培养基,在预定的时间点检测其还原度。 (责任编辑:大汉昆仑王)
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