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- 上海钰博
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- YB988Mu02
- 2025年07月14日
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- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Cytochrome P450 2E1 (CYP2E1)
- 库存：
10000
- 供应商：
钰博生物
细胞色素P450家族成员2E1(CYP2E1)重组蛋白
适用生物 Mus musculus (Mouse，小鼠)
细胞色素P450家族成员2E1(CYP2E1)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 29.4kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
ybG894Hu01 WNT1诱导信号通道蛋白2(WISP2)重组蛋白 Recombinant WNT1 Inducible Signaling Pathway Protein 2 (WISP2) Homo sapiens (Human)
ybG895Hu01 WNT1诱导信号通道蛋白1(WISP1)重组蛋白 Recombinant WNT1 Inducible Signaling Pathway Protein 1 (WISP1) Homo sapiens (Human)
ybG905Hu01 Vasorin蛋白(VASN)重组蛋白 Recombinant Vasorin (VASN) Homo sapiens (Human)
ybG915Hu01 泛醇细胞色素C还原酶核心蛋白Ⅰ(UQCRC1)重组蛋白 Recombinant Ubiquinol Cytochrome C Reductase Core Protein I (UQCRC1) Homo sapiens (Human)
ybG916Mu01 泛醌细胞色素C还原酶核心蛋白Ⅱ(UQCRC2)重组蛋白 Recombinant Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) Mus musculus (Mouse)
ybG917Hu01 尿嘧啶DNA糖基化酶(UNG)重组蛋白 Recombinant Uracil DNA Glycosylase (UNG) Homo sapiens (Human)
ybG918Hu01 尿调蛋白(UMOD)重组蛋白 Recombinant Uromodulin (UMOD) Homo sapiens (Human)
ybG919Hu01 尿苷二磷酸糖基转移酶8(UGT8)重组蛋白 Recombinant UDP Glycosyltransferase 8 (UGT8) Homo sapiens (Human)
ybG945Hu01 泛素羧基端酯酶L1(UCHL1)重组蛋白 Recombinant Ubiquitin Carboybl Terminal Hydrolase L1 (UCHL1) Homo sapiens (Human)
ybG948Hu01 泛醌蛋白2(UBQLN2)重组蛋白 Recombinant Ubiquilin 2 (UBQLN2) Homo sapiens (Human)
ybG962Mu01 翻译调节肿瘤蛋白1(TPT1)重组蛋白 Recombinant Tumor Protein, Translationally Controlled 1 (TPT1) Mus musculus (Mouse)
ybG963Hu01 胸苷酸合成酶(TYMS)重组蛋白 Recombinant Thymidylate Synthetase (TYMS) Homo sapiens (Human)
ybG966Hu01 干扰素α21(IFNα21)重组蛋白 Recombinant Interferon Alpha 21 (IFNa21) Homo sapiens (Human)
ybG971Hu01 干扰素α10(IFNα10)重组蛋白 Recombinant Interferon Alpha 10 (IFNa10) Homo sapiens (Human)
ybG979Hu01 谷氧还蛋白3(GLRX3)重组蛋白 Recombinant Glutaredoxin 3 (GLRX3) Homo sapiens (Human)
ybG981Hu01 谷氧还蛋白(GLRX)重组蛋白 Recombinant Glutaredoxin (GLRX) Homo sapiens (Human)
ybH006Hu01 丙糖磷酸异构酶1(TPI1)重组蛋白 Recombinant Triosephosphate Isomerase 1 (TPI1) Homo sapiens (Human)
ybH015Hu01 踝蛋白2(TLN2)重组蛋白 Recombinant Talin 2 (TLN2) Homo sapiens (Human)
适用生物 Mus musculus (Mouse，小鼠)
细胞色素P450家族成员2E1(CYP2E1)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 29.4kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Cytochrome P450 2E1 (CYP2E1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | RPA988Mu02 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 29.4kDa |
| Predicted isoelectric point | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | n/a |
| Residues | Pro34~Met273 (Accession # Q05421) with N-terminal His-Tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-PGPFPIP FFGNIFQLDL KDIPKSLTKL AKRFGPVFTL HLGQRRIVVL HGYKAVKEVL LNHKNEFSGR GDIPVFQEYK NKGIIFNNGP TWKDVRRFSL SILRDWGMGK QGNEARIQRE AHFLVEELKK TKGQPFDPTF LIGCAPCNVI ADILFNKRFD YDDKKCLELM SLFNENFYLL STPWIQAYNY FSDYLQYLPG SHRKVMKNVS EIRQYTLGKA KEHLKSLDIN CPRDVTDCLL IEM
MGHHHHHHSGSEF-PGPFPIP FFGNIFQLDL KDIPKSLTKL AKRFGPVFTL HLGQRRIVVL HGYKAVKEVL LNHKNEFSGR GDIPVFQEYK NKGIIFNNGP TWKDVRRFSL SILRDWGMGK QGNEARIQRE AHFLVEELKK TKGQPFDPTF LIGCAPCNVI ADILFNKRFD YDDKKCLELM SLFNENFYLL STPWIQAYNY FSDYLQYLPG SHRKVMKNVS EIRQYTLGKA KEHLKSLDIN CPRDVTDCLL IEM
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
细胞色素P450家族成员2E1(CYP2E1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
细胞色素P450家族成员2E1(CYP2E1)重组蛋白Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybG888Hu01 锚定蛋白重复域蛋白1(ANKRD1)重组蛋白 Recombinant Ankyrin Repeat Domain Protein 1 (ANKRD1) Homo sapiens (Human)
细胞色素P450家族成员2E1(CYP2E1)重组蛋白Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybG894Hu01 WNT1诱导信号通道蛋白2(WISP2)重组蛋白 Recombinant WNT1 Inducible Signaling Pathway Protein 2 (WISP2) Homo sapiens (Human)
ybG895Hu01 WNT1诱导信号通道蛋白1(WISP1)重组蛋白 Recombinant WNT1 Inducible Signaling Pathway Protein 1 (WISP1) Homo sapiens (Human)
ybG905Hu01 Vasorin蛋白(VASN)重组蛋白 Recombinant Vasorin (VASN) Homo sapiens (Human)
ybG915Hu01 泛醇细胞色素C还原酶核心蛋白Ⅰ(UQCRC1)重组蛋白 Recombinant Ubiquinol Cytochrome C Reductase Core Protein I (UQCRC1) Homo sapiens (Human)
ybG916Mu01 泛醌细胞色素C还原酶核心蛋白Ⅱ(UQCRC2)重组蛋白 Recombinant Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) Mus musculus (Mouse)
ybG917Hu01 尿嘧啶DNA糖基化酶(UNG)重组蛋白 Recombinant Uracil DNA Glycosylase (UNG) Homo sapiens (Human)
ybG918Hu01 尿调蛋白(UMOD)重组蛋白 Recombinant Uromodulin (UMOD) Homo sapiens (Human)
ybG919Hu01 尿苷二磷酸糖基转移酶8(UGT8)重组蛋白 Recombinant UDP Glycosyltransferase 8 (UGT8) Homo sapiens (Human)
ybG945Hu01 泛素羧基端酯酶L1(UCHL1)重组蛋白 Recombinant Ubiquitin Carboybl Terminal Hydrolase L1 (UCHL1) Homo sapiens (Human)
ybG948Hu01 泛醌蛋白2(UBQLN2)重组蛋白 Recombinant Ubiquilin 2 (UBQLN2) Homo sapiens (Human)
ybG962Mu01 翻译调节肿瘤蛋白1(TPT1)重组蛋白 Recombinant Tumor Protein, Translationally Controlled 1 (TPT1) Mus musculus (Mouse)
ybG963Hu01 胸苷酸合成酶(TYMS)重组蛋白 Recombinant Thymidylate Synthetase (TYMS) Homo sapiens (Human)
ybG966Hu01 干扰素α21(IFNα21)重组蛋白 Recombinant Interferon Alpha 21 (IFNa21) Homo sapiens (Human)
ybG971Hu01 干扰素α10(IFNα10)重组蛋白 Recombinant Interferon Alpha 10 (IFNa10) Homo sapiens (Human)
ybG979Hu01 谷氧还蛋白3(GLRX3)重组蛋白 Recombinant Glutaredoxin 3 (GLRX3) Homo sapiens (Human)
ybG981Hu01 谷氧还蛋白(GLRX)重组蛋白 Recombinant Glutaredoxin (GLRX) Homo sapiens (Human)
ybH006Hu01 丙糖磷酸异构酶1(TPI1)重组蛋白 Recombinant Triosephosphate Isomerase 1 (TPI1) Homo sapiens (Human)
ybH015Hu01 踝蛋白2(TLN2)重组蛋白 Recombinant Talin 2 (TLN2) Homo sapiens (Human)
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文献和实验相关实验
肝细胞的标志——醛缩酶 B mRNA 的水平，达到新鲜分离的人肝细胞 20% 的水平。相比 HepaG2 细胞，其白蛋白和触珠蛋白 mRNA 的表达水平比 HepaRG 细胞低，检测不到醛缩酶 B 的转录。二、药物代谢相关酶系细胞色素 P450（CYP）是最大的药物代谢酶蛋白超家族之一，其中参与人体肝药物代谢的主要有 CYP1、CYP2、CYP3 及 CYP4 家族。P450 酶易受多种内外源性物质的诱导或抑制，导致酶数量和活性改变，从而改变药代动力学，使药物活性物质累积导致毒性增强或降低其血
作用等方面具有重要意义。 1.3 CYP450酶及代谢表型研究方法 CYP450为一类含亚铁血红素蛋白的超家族，根据相关酶亚型在药物代谢中的重要程度，可将CYP450分为以下三类：（1）主要CYP450，包括CYP1A2、CYP2C9 、CYP2C19 、CYP2D6 和CYP3A4；（2）较主要CYP450，包括CYP2B6、CYP2C8和CYP3A5；（3）次要CYP450，包括CYP1A1、CYP1B1、CYP2A6、CYP2E1、CYP2J2和CYP4A11等。参与药物代谢的动物肝微粒
450 为一类含亚铁血红素蛋白的超家族，根据相关酶亚型在药物代谢中的重要程度，可将 CYP450 分为以下三类：（1）主要 CYP450，包括 CYP1A2、CYP2C9 、CYP2C19 、CYP2D6 和 CYP3A4；（2）较主要 CYP450，包括 CYP2B6、CYP2C8 和 CYP3A5；（3）次要 CYP450，包括 CYP1A1、CYP1B1、CYP2A6、CYP2E1、CYP2J2 和 CYP4A11 等。参与药物代谢的动物肝微粒体 P450 酶较为复杂，而人肝微粒体中参与药物
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细胞色素P450家族成员2E1(CYP2E1)重组蛋白
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