丝裂原激活蛋白激酶激酶1(MAP2K1)重组蛋白
适用生物 Homo sapiens (Human,人)
丝裂原激活蛋白激酶激酶1(MAP2K1)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 39.0kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Mitogen Activated Protein Kinase Kinase 1 (MAP2K1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species |
Homo sapiens (Human) |
Product No. |
RPD559Hu01 |
Source |
Prokaryotic expression |
Host |
E.coli |
Purity |
> 95% |
UOM |
50ug |
Predicted Molecular Mass |
39.0kDa |
Predicted isoelectric point |
7.0 |
Applications |
SDS-PAGE; WB; ELISA; IP. |
Endotoxin Level |
<1.0EU per 1µg (determined by the LAL method) |
Subcellular Location |
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome |
Residues |
Leu54~Val369 (Accession # Q02750) with two N-terminal Tags, His-tag and T7-tag |
Formulation |
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LTQKQKV GELKDDDFEK ISELGAGNGG VVFKVSHKPS GLVMARKLIH LEIKPAIRNQ IIRELQVLHE CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS LDQVLKKAGR IPEQILGKVS IAVIKGLTYL REKHKIMHRD VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY SVQSDIWSMG LSLVEMAVGR YPIPPPDAKE LELMFGCQVE GDAAETPPRP RTPGRPLSSY GMDSRPPMAI FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA DLKQLMVHAF IKRSDAEEV
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
丝裂原激活蛋白激酶激酶1(MAP2K1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
丝裂原激活蛋白激酶激酶1(MAP2K1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-3011R phospho-Bcl 10 (Ser218)磷酸化Bcl-10抗体 浓度1mg/ml
yb-3012R phospho-Bim(Ser87)磷酸化细胞死亡调解子抗体 浓度1mg/ml
yb-3013R phospho-B-Raf (Ser365)磷酸化B-Raf抗体 浓度1mg/ml
yb-3014R Phospho-BRCA1 (Ser1524)磷酸化乳腺癌易感基因1抗体 浓度1mg/ml
yb-3750R BMAL1芳香烃受体核转录蛋白样1抗体 浓度1mg/ml
yb-6888R BRCC3乳腺癌易感基因复合物亚基蛋白3抗体 浓度1mg/ml
yb-12418R BAMBI肿瘤转移抑制蛋白BAMBI抗体 浓度1mg/ml
yb-12393R BCL9B淋巴细胞淋巴瘤因子9抗体 浓度1mg/ml
yb-12868R Biliverdin Reductase胆绿素还原酶抗体 浓度1mg/ml
yb-12869R BIVMBIVM蛋白抗体 浓度1mg/ml
yb-12870R Blood Group Lewis a血型Lewis A抗原抗体 浓度1mg/ml
yb-12871R Blood Group Lewis b粘蛋白/岩藻糖基转移酶3抗体 浓度1mg/ml
yb-12872R BLMBloom综合征相关蛋白抗体 浓度1mg/ml
yb-12873R BMP8b骨形态发生蛋白8b抗体 浓度1mg/ml
yb-12874R phospho-BMX (Tyr566)磷酸化非受体性蛋白酪氨酸激酶ETK抗体 浓度1mg/ml
yb-12875R phospho-BNIP3 (Ser95)磷酸化促凋亡调节蛋白BNIP3抗体 浓度1mg/ml
yb-12876R BOB1B淋巴细胞特异性激活OCT结合蛋白1抗体 浓度1mg/ml
yb-12877R BOP1核糖体合成蛋白BOP1抗体 浓度1mg/ml
yb-12878R BORA调节中心体分离早期相关激酶BORA抗体 浓度1mg/ml
yb-12879R Borrelia burgdorferi莱姆病螺旋体抗体 浓度1mg/ml
yb-12880R BOULE精子成熟相关蛋白BOULE抗体 浓度1mg/ml
yb-12881R BPOZ伸长因子结合蛋白抗体 浓度1mg/ml
yb-12882R BPY2睾丸特异性碱性蛋白Y2抗体 浓度1mg/ml
yb-12883R BRAF35乳腺癌易感基因2相关蛋白抗体 浓度1mg/ml
yb-12884R Phospho-BRCA1(Ser1280)磷酸化乳腺癌易感基因1抗体 浓度1mg/ml