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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
6个月
- 英文名:
Recombinant Mothers Against Decapentaplegic Homolog 1 (Smad1)
- 库存:
10000
- 供应商:
钰博生物
- 规格:
50ug
适用生物 Homo sapiens (Human,人)
Smad同源物1(Smad1)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 n/a 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
| Organism species | Homo sapiens (Human) |
| Product No. | RPA647Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Predicted isoelectric point | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | n/a |
| Residues | n/a |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Smad同源物1(Smad1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验【求助】请问激活SMAD通路,samd表达上升还是其磷酸化?
量增加的话,其激活也有可能增加,因为smad2/3磷酸化的绝对量可能增加。3.MIRNA下降是使samd2/3磷酸化还是表达上升? 没明白这句话是什么意思,能详细一点吗? fpinzaghi 请问你做Smad1/5/8么,总蛋白是不是用Smad1? 本文由丁香园论坛提供,想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你,都可以成为师兄的好伙伴 师兄微信号:shixiongcoming
proteins (Smad1, Smad5, and Smad8), which provides a way to monitor overall pathway activity in the mammalian embryonic kidney.
constitutively overexpressing the BMP antagonist noggin, did not differentiate into cardiomyocytes. Cooverexpression of Smad1, a ligand-specific Smad, and Smad4, a common Smad, restored the ability of P19CL6noggin to differentiate into cardiomyocytes
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