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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名:
KeMin bio
- 供应商:
上海恪敏生物
- 规格:
10 mL
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文献和实验除>95%的蛋白提取和样品制备过程中所使用的去垢剂,而蛋白和多肽损失极少。此树脂适用于常用去垢剂的去除,如浓度在0.5-1%的SDS、Triton X-100、NP-40和CHAPS。目前有预装的离心柱和离心板形式,最多可处理100 µL样品。另外,您也可以购买HiPPR Detergent Removal Spin Column Kit,其中包含树脂浆和空的离心柱,便于您自己调整样品体积。 整个处理过程相当简单,仅需15分钟。首先是离心去除保存液,之后用缓冲液平衡树脂并离心,重复
Enrichment of Membrane Proteins by Partitioning in Detergent/Polymer Aqueous Two-Phase Systems
, which is similar to that of the anionic detergent sodium dodecyl sulfate (SDS) but with the important advantage of being nondenaturing. Detergent/polymer two-phase system partitioning offers removal of soluble proteins that can be further improved by manipulation
protein complexes. Separation methods have been developed based on nondenaturing detergent extraction of yeast mitochondrial membrane proteins followed by enrichment of hydrophobic proteins in aqueous two-phase system. Combining the zwitterionic detergent
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