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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
KeMin bio
- 供应商:
上海恪敏生物
- 规格:
6/10 mL
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文献和实验Detergent-Extracted Models for the Study of Cilia or Flagella
in inducing motion but not coordinated swimming. Applying the glycerination technique to sea urchin sperm, Brokaw and Benedict (4 ) successfully restored coordinated beating of the flagellum and progressive swimming. Using the nonionic detergent Triton X-100
The Isolation of Detergent-Resistant Lipid Rafts for Two-Dimensional Electrophoresis
biochemical properties such as Triton X-100 insolubility and low density. To solubilize completely the proteins embedded in lipid rafts, sample lysis buffer (9 M urea, 2 M thiourea, 100 mM DTT, 2% CHAPS (w/v), 60 mM n -octyl -D-glucopyranoside, 2% IPG buffer
proteins were resistant to solubilization by nonionic detergents such as Triton X-100 (1 ). This detergent insolubility is acquired as the proteins pass through the endoplasmic reticulum and on to the Golgi apparatus (2 ), and arises not from a direct
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