低钙（0.02 gm%）鼠粮 / Modified D100

In Situ Hybridization Using Digoxigenin Labeled Probes

. The staining reaction can be observed by blotting off excess solution and examining the slide under the microscope using a low magnification phase contrast lens (e.g. 16x) 9) Stop the reaction by dipping the slides in distilled water three times

Calcium Phosphate Transfection of Neurons in Primary Culture

  Calcium Phosphate Transfection of Neurons in Primary Culture Developed by Hank Dudek and Zhengui Xia (11/96) Modified by Steve Finkbeiner (rev. 7/97) Departments of Neurology and Physiology, UCSF/GIND 415-695-3868

DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE

(can vary from 5-35 mM) 35 mM potassium ferricyanide (can vary from 5-35 mM) 2 mM MgCl2 0.02% Nonidet P-40 (NP-40) (diluted from 10% stock solution) 0.01% Na deoxycholate (diluted from 10% stock solution) in PBS X-gal