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- 宿主:
NA
- 适应物种:
Human
- 克隆性:
单克隆
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文献和实验Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
min after the seeding and then every 1 h for a period of 96 h. Recombinant TGF-β1 (Millipore) treatment with various concentrations in triplicate was performed 24 h after the seeding of the cells. Formation of contractile microfilaments was blocked
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
/) 96‐well plate (8 well removable strips) coated with polyclonal rabbit anti‐PINP antibody (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/) Precision
人 Lewisa 单克隆抗体(Calbiochem, Meudon, France) ( 保存于 -20℃)。 ( 6 ) TTBS 缓冲液:20 mmol/L Tris-HCl,pH 7.4,含 0.5 mol/L NaCl 和 0.1% Tween -20。 ( 7 ) 羊抗鼠免疫球蛋白辣根过氧化酶标记抗体(Sigma Aldrich) ( 保存于 -20°C ) 。 ( 8 ) 羊抗兔免疫球蛋白辣根过氧化酶标记抗体(Bio- Rad ) ( 保存于 2~8°C
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