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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
2-3周
- 供应商:
研卉生物
- 检测范围:
520 nm
- 检测方法:
流式 荧光
- 应用:
流式 荧光
- 样本:
细胞 组织
- 规格:
200T
总活性氧(reactive oxygen species,ROS)检测试剂盒(200)520 nm的试验
活性氧ROS检测试剂盒 促销价:2266元/200T
活性氧检测试剂盒说明书
RUO: For Research Use Only. Not for use in diagnostic procedures.
Description: The Total Reactive Oxygen Species (ROS) Assay Kit 520 nm contains the necessary regent and buffer for identifying ROS in cells by flow cytometry in the FITC channel. Reactive oxygen species are chemically reactive oxygen-containing molecules that are generated as a natural byproduct of the oxygen metabolism. ROS are constantly generated and eliminated under normal physiologic conditions and have important functions in cell signaling, homeostasis, and clearance of microbial infections. During times of environmental stress such as exposure to UV or heat, or during infection, ROS levels can increase dramatically leading to oxidative stress. Oxidative stress can result in damage to cellular proteins, lipids, and DNA, and has been linked to cardiovascular disease, cancer, diabetes, inflammation, and aging. Examples of ROS include superoxide ions and peroxides.
活性氧检测试剂盒参考文献 References: Freemerman AJ, Johnson AR, Sacks GN, Milner JJ, Kirk EL, Troester MA, Macintyre AN, Goraksha-Hicks P, Rathmell JC, Makowski L. Metabolic reprogramming of macrophages: glucose transporter (GLUT1)-mediated glucosemetabolism drives a pro- inflammatory phenotype. J Biol Chem. 2014 Feb 3.
Kramer PA, Ravi S, Chacko B, Johnson MS, Darley-Usmar VM. A review of the mitochondrial and glycolytic metabolism in human platelets and leukocytes: Implications for their use as bioenergetic biomarkers. Redox Biol. 2014 Jan 10;2:206-210.
Miletic AV, Graham DB, Montgrain V, Fujikawa K, Kloeppel T, Brim K, Weaver B, Schreiber R, Xavier R, Swat W. Vav proteins control MyD88-dependent oxidative burst. Blood. 2007 Apr 15;109(8):3360-8.
| Components | ROS Assay Stain Concentrate (lyophilzed powder): 1 x vial; store at less than or equal to -20 C. ROS Assay Buffer: 1 x 20 mL bottle; store at less than or equal to -20 C. |
|---|---|
| Reported Applications | Flow Cytometric Analysis |
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文献和实验一、反应曲线 首先,我们了解一下化学反应的时间与吸光度一个曲线图,可以划分为四个区:延迟区、等速区、过渡区和平衡区,如下图所示: 二、反应原理 对于延迟区来讲,无规律可寻,对于指标检测没有意义。 等速区对应的指标检测方法是速率法。速率法又称连续监测法,是在测定酶活性或用酶法测定代谢产物时,连续选取时间-吸光度曲线中线性期内4个以上测光点作为读数点,并以单位时间吸光度变化值计算结果。线性期就是此期间内各测光点之间的吸光度差值相等,此线性期对酶促反应的底物来说属于零级反应。其测光点一般设置在加入
宽度:750 px高度:422 px维持原图长宽比:确认 ningwang 我在做关于细胞活性氧浓度的测定,来探讨药物对细胞的保护作用。买的是碧云天生成的活性氧检测试剂盒,但是不知道胞内的荧光用什么仪器可以检测得到,望各位指导。 伽利略 流式细胞仪 实际上这些问题你可以发信直接问试剂公司。 哈啰 激光共聚焦显微镜、荧光分光光度计、荧光酶标仪或流式细胞仪都可以的,试剂盒里说明书上有写的,个人
、加冰水等生物或者非生物刺激,均可以诱导细胞ROS的快速产生。 你这个作用强度不好控制。 baby1_9253344 ecust小熊 wrote: 你这个作用强度不好控制。 我外行了,是不是只要不适合细胞的生长环境都会刺激生成ROS? ikewei ROS 即活性氧,具体的定义教科书上,网上大把,就不在说了。使细胞中的ROS升高的因素
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