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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Phospho-AKT1 (Ser473) Monoclonal Antibody (104A282)
- 浓度:
1 mg/mL
- 宿主:
Mouse
- 供应商:
嵘崴达
- 库存:
需确认
- 保质期:
见产品外包装
- 克隆性:
单克隆
- 保存条件:
4°C
- 亚型:
IgG1
- 规格:
50 µg
SulfoLink™ 固定试验试剂盒,2 mL
货号: 20325
Thermo Scientific SulfoLink 固定试剂盒(适用于蛋白质,2 mL)包含用于制备蛋白质样品进行有效固定的所有试剂,以及五个含有 2 mL SulfoLink 树脂并可以重力流或离心管形式用于进行高效耦合反应和多个周期亲和纯化的的色谱柱。
SulfoLink 固定试剂盒(适用于蛋白质,2 mL)的特点:
•巯基 (-SH) 基团特异性—碘yi酸基团与巯基发生特异性反应,形成不可逆的硫醚键
• 快速—离心柱可提高方案速度;在 3.5 小时内制备和偶联蛋白质样品
• 灵活的偶联条件—可根据偶联反应期间的蛋白质或肽溶解度需求,使用 pH 为 7.5 至 9.0 的水性缓冲液、有机溶剂(例如 20% DMSO)或变性剂(盐酸·胍)
• 简单易懂的说明—简化的样品制备、固定化和亲和纯化方案
• 高容量—每 2mL SulfoLink 耦合树脂柱可固定 2 至 20mg 蛋白质
应用:
• 固定含末端半胱氨酸残基的各种肽以纯化由通过马来酰亚胺偶联制备的肽免疫原生成的各种抗体
•通过铰链区巯基,以定向方式固定各种抗体以确保抗原结合位点没有受到位阻,可进行抗原亲和纯化
| 20325 | SulfoLink™ 固定试验试剂盒,2 mL | 1 column |
| 20333 | Pierce Protein A Agarose | 5 mL |
| 20334 | Pierce Protein A Agarose | 25 mL |
| 20339 | Detoxi-Gel Endotoxin Removing Gel | 10 mL |
| 20340 | Detoxi-Gel Endotoxin Removing Gel | 1000 mL |
| 20341 | Immobilized Papain | 5mL |
| 20343 | Pepsin Agarose | 5 mL |
| 20344 | Detoxi-Gel Endotoxin Removing Columns, 1 mL | 5 X 1 mL |
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
kinase is KIR 3.1 and have developed a phospho-selective antibody against the Y12 motif of this channel. Much like KOR and p38 MAP kinase, phosphorylation of this potassium channel increases following repeated stress. The following chapter discusses
largely independent of the surrounding amino-acid sequence. The antibody is phospho-specific but does not cross-react with phospho-serine-containing sequences. The anti-phosphothreonine monoclonal antibody is labeled with Eu3+ Cryptate by Cisbio
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