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南京赛泓瑞
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文献和实验Protein Purification: Assays, Specific Activity, Initial Fractionation
The nitrocellulose is then incubated with a second antibody which is specific for the first antibody. For example, if the first antibody was raised in rabbits, the second antibody might be termed "goat anti-rabbit immunoglobulin". What this means is that rabbit
x stock Tris/ Glycine SDS buffer were from National Diagnostics, (Hull, UK).eNOS (NOS 3) affinity purified rabbit polyclonal antibody was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, Calafornia, USA).Goat Anti-Rabbit horseradish peroxidae
population is termed monoclonal . It recognizes a single epitope on the antigenic protein. Antibody producing B lymphocytes can be isolated from the spleen or from lymph nodes. However, they have a finite life span in culture, i.e
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