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- 详细信息
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westang
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文献和实验solution 3. Incubate with secondary antibody diluted in Blocking buffer for 60 min at room temp. 4. 3 x10min secondary washing solution 1 x10min alkaline phosphatese buffer 5. Detect with Chromogenic I. Stripping blot 1. Rinse blot off with 0.05
. Lysis buffer (store on the benchtop without proteinase K). 60 g/ml proteinase K 10mM Tris-Cl, pH 8.2 50mM KCl 2.5mM MgCl2 0.45% Tween-20 0.05% gelatin Master mix . Prepare a master mix containing the following
. Lysis buffer (store on the benchtop without proteinase K). 60 g/ml proteinase K 10mM Tris-Cl, pH 8.2 50mM KCl 2.5mM MgCl2 0.45% Tween-20 0.05% gelatin Master mix . Prepare a master mix containing the following
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