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- B1020
- Amresco分装
- 2026年08月27日
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文献和实验it self is very similar to a standard CaCl2 transformation. Place 100 µl of TB buffer in a tube on ice. Melt the ligation mix at 65℃ for 5 minutes and add a 5-15 µl aliquot of the ligation mix to the TB solution and vortex quickly. Add 200 µl of competent
DNA Recovery With Low Melt Agarose
Recovery of DNA from Low Melting Point Agarose Gels 1.Run digestion products on 0.7% LMP agarose gel in 1X TBE (it's nice to have at least 1ug of the fragment you want). LMP agarose is fragile; pour gel with EtBr 2.Let solidify in cold room
competent cells (DH5alpha at competency of 108 cfu/µl of pUC18). The transformation procedure it self is very similar to a standard CaCl2 transformation. Place 100 µl of TB buffer in a tube on ice. Melt the ligation mix at 65℃ for 5 minutes and add
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