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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
西宝生物
- 库存：
50TEST
- 保存条件：
-20℃
- 英文名：
TA-BLUNT LIGATION KIT
- 规格：
100tests
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文献和实验This protocol uses Promega''s pGEM-T kit (#A3600). PCR For TA cloning, it is optimal if the PCR primers have G''s at the 5'' end as this will maximize the probability of Taq polymerase adding the terminal A overhang
ligation of the vector without any insert. If the background is low, there is no need for screening of the transformed cells for vector with ligated insert. c) For blunt end ligation (which is much less efficient compare to cohesive end ligation
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
-end ligation (1 ). The Invitrogen (Carlsbad, CA) TA Cloning� Kit combines the efficiency of cohesive-end ligation with the ease of direct cloning of PCR products. A sample may be removed from the completed PCR reaction and transferred directly
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