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文献和实验Simplified Calcium Phosphate Coprecipitation
Materials 2x HEPES: 8 g NaCl, 0.105g NaHPO4 , 6.5 g HEPES, H2O to 500 mL, pH 6.95 to 7.10 (try a range) 2M CaCl2 : store in aliquots at -20°C DNA 4 mg/mL Procedure (The volumes can be scaled
DNA Transfection: Calcium Phosphate Method
The calcium phosphate transfection is a widely used method for introducing foreign DNA plasmids into cells. Mechanisms underlying this transfection method are not yet defined; however, DNA–calcium phosphate precipitates are internalized
Characterization of a Calcium Phosphate-Based Matrix for rhBMP-2
injectable carrier to deliver rhBMP-2 percutaneously to closed fractures. Here, we outline characterization of a calcium phosphate (CaP)-based injectable carrier as a delivery matrix for rhBMP-2.
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