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文献和实验for a decrease of fluorescence lifetime. Total enhancement is the product of these two effects. The recent reviews (14 , 15 ) contain more details on MEF. 2. Model Immunoassays To demonstrate a metal-enhanced fluorescence immunoassay on the glass
Measurement with the Light Microscope
Conversion factor Identify the ocular micrometer. A typical scale consists of 50 - 100 divisions. You may have to adjust the focus of your eyepiece in order to make the scale as sharp as possible. If you do that, also adjust the other eyepiece to match
PREPARATION OF MICROINJECTION PIPETTES
H20. Drain well. 4. Cover cylinder with parafilm, poke holes in it using a needle and place glass in 60¡C oven until dry. This takes a few days. Pipette pulling:- Flamming Brown micropipette puller Model P-87 Sutter Instrument Co. USA
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