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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
西宝生物
- 库存：
大量
- 规格：
.2ML
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文献和实验Synchronizing Protein Transport in the Secretory Pathway
(see recipe ) Primary antibody against streptavidin (e.g., monoclonal anti‐Streptavidin clone S10D4) Secondary antibody coupled
Wholemount staining of embryos
as above 15 to 30''. Secondary antibody is added in blocking solution. Concentration varies, but 1/500 works for most. For monoclonal primaries use anti-mouse coupled to AP or HRP, for polyclonals use anti-rabbit coupled AP or HRP. Incubate at 4 degC
confirmation. A. Protein microarray hybridization with 8F1. The OriGene overexpression protein microarray chip was immunostained with the most commonly used 8F1 monoclonal anti-ERCC1 antibody. The positive reactive proteins are highlighted with red arrows
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