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文献和实验Transient Transfection of Cos-1 Cell
to, cell density, transfection reagent, duration of transfection, and DNA concentration.1. Seed a six-well tissue culture plate with 1-2 x 105 Cos-1 cells in 2ml of DMEM supplemented with 10% fetal calf serum. Incubate for 18-24 hours at 37¼C in a CO2
reagent, duration of transfection, and DNA concentration. 1. Seed a six-well tissue culture plate with 1-2 x 105 Cos-1 cells in 2ml of DMEM supplemented with 10% fetal calf serum. Incubate for 18-24 hours at 37¼C in a CO2 humidified incubator
Transient Transfection of Cos-1 Cells
, but are not limited to, cell density, transfection reagent, duration of transfection, and DNA concentration. 1. Seed a six-well tissue culture plate with 1-2 x 105 Cos-1 cells in 2ml of DMEM supplemented with 10% fetal calf serum. Incubate for 18-24 hours at 37
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