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文献和实验E-Z 96® Viral RNA Protocol with Centrifugation
试剂 Materials supplied by user 1. 96-100% ethanol 2. β-Mercaptoethanol 实验设备 Materials supplied by user 1. Multichannel pipet 2. RNase-free filter pipette tips 3. Reagent reservoirs
Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates
2 times with PBS. 4. Using the multi-channel pipetter, add 50 ul of trypsin to each of the wells. Change pipette tips between wells! 5. Incubate @ 37o C for 10-15 minutes. 6. Add 50 ul of 2X Freezing Media (60% DMEM, 20% FCS, 20
Gene Knockout Techniques(1) The Knockout Process
of sterile M9 buffer and letting the plate sit overnight. It helps to make the buffer up to 0.01% Triton-X-100 (100 ul in 1 liter), which doesn?t hurt the worms and keeps them from sticking to the plastic of tips and centrifuge tubes. In either procedure
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