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文献和实验Western Blotting with Horseradish Peroxidase Conjugates
7.5, 100mM NaCl, 0.1% Tween 20). Incubate the blot for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C. INCUBATION WITH HORSERADISH PEROXIDASE CONJUGATED ANTIBODY NOTE: THE INCLUSION OF SODIUM AZIDE IS TO BE AVOIDED
Lignin-Degrading Enzyme Activities
lignin peroxidase (with a relatively high redox potential), manganese peroxidase, an alkyl aryl etherase, and laccase. The peroxidases and laccase, but not the etherase, are expressed extracellularly by white-rot fungi. A number of these microorganisms
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
in deionized water. 12. Cover the sections with 2% aqueous solution of BSA for 10 min at room temperature. 13. Wash in deionized water. 14. Immerse in PBS for 5 min. 15. Cover the sections with Extra-avidin Peroxidase diluited 1:10-1:20 in water
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