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- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
咨询甄准
- 保质期：
咨询甄准
- 英文名：
Dried Aluminum Hydroxide Gel (200 mg)
- 库存：
10
- 供应商：
上海甄准
- CAS号：
21645-51-2
- 规格：
咨询甄准
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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
instead of TE since the EDTA inhibits Taq Polymerase. 12、The DNA concentration then is estimated by analysis of 10 ul of the 210 ul final DNA solution by agarose gel electrophoresis and by measuring the A260 in the spectrometer. Typical BAC yields per 200 ml
In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
4. In-Gel digestion with Trypsin Precool tubes with the dried gel particles and the Trypsin solution on ice Rehydrate the gel particles in the trypsin solution (12.5 ng/µl Trypsin [Promega #V5111] in 50 mM NH4HCO3) for 30-45 minutes at 4°C. Add
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