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文献和实验GLASS BEADS 0.45 - 0.5mm beads (Sigma) Clean by soaking in nitric acid -->stir bar, O/N.** **Note, dispose of acid in appropriate flask. Wash w/ lots distilled water
Acid Washing Beads【Yale University 】
Acid Washing Beads Peter Novick Lab, Department of Cell Biology Yale University School of Medicine http://info.med.yale.edu/cellbio/Novick/Second/Protocols/AcidBeads.pdf Materials: 1) 0.5 mm glass beads (Biospec Products) 1-4 lbs 2) 1% Triton
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
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