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- 2025年10月31日
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
) (for Protocols 2-4) Recombinant murine IL-7 (Peprotech 217-17) (for Protocols 2-4) Trypsin solution, 0.25% (Invitrogen 15090046) (for Protocols 1, 2) Dilute stock trypsin to 0.25% with PBS. Equipment Biosafety cabinet Cell strainers (40-µm pore size
【资源】Gelatin Zymography Protocol
of 0.5% (w/v) instead of the usual concentration of 0.1%. Gels should be destained with an appropriate Coomassie R-250 destaining solution (Methanol : Acetic acid : Water (50 : 10 : 40). Areas of protease activity will appear as clear bands against a dark
【资源】Protocol of Gelatin Zymography
contrast, use a stain concentration of 0.5% (w/v) instead of the usual concentration of 0.1%. Gels should be destained with an appropriate Coomassie R-250 destaining solution (Methanol : Acetic acid : Water (50 : 10 : 40). Areas of protease activity
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