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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
trans-1,2-Dichloroethylene Standard Solution(1mg/m
- 库存：
大量
- 供应商：
FUJIFILM Wako
- 规格：
2mL
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文献和实验Standard hybridization technique
sulphate (Sigma) Standard hybridisation solution: 6 x SSC,500μg/ml heparin,1% SDS. N.B: A modification used for the hybridisation buffer which can also give extremely good results is a variation on that described by Church and Gilbert
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
. autoclave, cool, and add: 1x MS vitamines (SIGMA #M-7150). Take 1ml of 1000x stock prepared by disolving 10.3gr in 100ml of water. antibiotic (kanamycin 50mg/l). Top agar: 1x Murashige&Skoog salts. 1% sucrose. adjust pH
Standard hybridization technique
). You will need: 6 x SSC (0.9M NaCl,90mM sodium citrate,pH 7) 10% SDS 50mg/ml heparin sulphate (Sigma) Standard hybridisation solution: 6 x SSC,500μg/ml heparin,1% SDS. N.B: A modification used for the hybridisation buffer which can also give extremely good results
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