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- 英文名：
Copper(II) Sulfate Pentahydrate
- 供应商：
富士胶片和光
- 规格：
25G
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文献和实验Extraction of the Functional Manganese and Calcium From Photosystem II
spectroscopic measurements between intact and metal-depleted samples. In this chapter, we outline three experimental procedures used for the various removal of Mn and Ca from photosystem (PS) II-containing (i.e,. O2 -evolving) preparations: the complete Mn
PulseChase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading
. Proteins of interest are isolated (usually by immunoprecipitation) and resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the fate of radiolabeled molecules is examined by autoradiography. This chapter describes a pulse–
Bicinchoninic Acid (BCA) Protein Assay (Smith)
below. Principle BCA serves the purpose of the Folin reagent in the Lowry assay,namely to react with complexes between copper ions and peptide bonds to produce a purple end product.The advantage of BCA is that the reagent is fairly stable under alkaline conditions
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