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- 文献和实验
- 技术资料
- 保存条件:
25℃以下
- 英文名:
Bismuth Standard Solution
- 库存:
大量
- 供应商:
FUJIFILM Wako
- CAS号:
N/A
- 规格:
100 ml
Bismuth Standard Solution
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文献和实验Standard hybridization technique
The standard solution typically used for both pre-hybridisation and hybridisation is based
as otherwise all available Mg2+ is chelated out of solution and enzyme reactivity is adversely affected. Any increase in dNTPs over 200uM means [Mg2+] should be re-optimised.AVOID USING EDTA-CONTAINING BUFFERS AS EDTA CHELATES Mg2+Low primer, target, Taq, and nucleotide
Standard hybridization technique
The standard solution typically used for both pre-hybridisation and hybridisation is based on that given in Maniatis et al.,(1982)with both Denhart's solution and heterologous DNA being replaced by heparin (Singh and Jones,1984
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