氨基丁酸转运蛋白VGAT抗体产品WB实验中的对照系统:
he chromogenic reaction (Note: two anti generally have two kinds of commonly used marker enzyme, HRP and AP, chromogenic substrate kit and its corresponding to DAB and BCIP/NBT, the general use of chemiluminescence lysate.) Westernbloting is the first to transfer after electrophoresis separation of proteins from the gel onto a NC membrane, there are generally two ways: capillary blotting and electrophoretic blotting. Westernbloting is a common method used to detect the protein, through the immune reaction of antigen antibody can be quantitatively measured the relative molecular weight of antibody, and the relative abundance of known antigen or antibody and the relation between. At present, it is applied in many fields, detection of infectious diseases and autoimmune diseases, allergies, etc. in clinic, in antibody production field it with ELISA, ICC, IHC, IFA and other immune technology has become the main means of engaging a screening of monoclonal antibody production.
WB对照系统
阳性对照:最好有标准品,或阳性血清、阳性上清。
阴性对照:测血时用相应小鼠未免疫血清,测培养上清,用无克隆培养上清。
空白对照:不加一抗,用1%BSA代替。
无关对照(替代对照):用无关项目一抗,或无关项目的抗体。