- ￥100 - 9999
- Thermo Fisher Scientific
- 37570
- 美国
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
KeMin bio
- 供应商：
上海恪敏生物
- 规格：
1 L
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文献和实验Protein Syntheses in Cell Free Systems
/v) glycerol 1X TBS-M buffer containing 0.25 M sucrose 1X TBS-M buffer containing 1.0 M sucrose Sephadex G-25 column equilibrated with 1X TBS-M buffer Liquid nitrogen storage Reaction mixture for protein synthesis
Protein Syntheses in Cell Free Systems
equilibrated with 1X TBS-M buffer•Liquid nitrogen storage•Reaction mixture for protein synthesis,containing the following in a total volume of 50 µl 2Tris-HCl,pH 7.5 1.5 µmMg acetate 0.15 - 0.20 µmKCL 4.0 - 5.0 µm-Mercaptoethanol0.25 µmATP 0.05 µmGTP 0.005 µ
Blocking of unwanted non-specific staining
Blocking endogenous biotin Can be done with commercially available kits or by buying the isolated components of the kits, free biotin and free avidin. Can also be done with simpler reagents as below. 1- Wash twice in TBS 0.05M pH
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