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文献和实验Calpain Zymography with Casein or Fluorescein Isothiocyanate Casein
, and a useful modification is to use fluorescein isothiocyanate (FITC)-casein in place of casein in the gels. The FITC-casein system is also more sensitive. In this case, the gels are viewed and photographed with ultraviolet (UV) illumination, and proteinase
Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin and for unlabeled biotin in homogeneous fluorescence assays. The primary standard is a 16 μM working solution of d-biotin which is used to titrate an aliquot
Functionality Screen of Streptavidin Mutants by Non-Denaturing SDSPAGE Using Biotin-4-Fluorescein
screening methodologies in mind, it is imperative to detect, prior to the high-density protein production, the bacteria that produce non-functional streptavidin isoforms. Based on the incorporation of biotin-4-fluorescein in streptavidin mutants present
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