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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
1gG
- 保存条件:
2-8℃ 短期保存或<-20℃长期保存保存1年以上
- 克隆性:
多克隆
- 标记物:
电询
- 适应物种:
hu, mo, rat
- 宿主:
电询
- 应用范围:
电询
- 浓度:
1mg/1ml
- 靶点:
电询
- 抗体英文名:
Goat Anti-Mouse IgG/Cy5
- 抗体名:
Cy5标记的羊抗小鼠IgG
- 规格:
0.1ml
中文名称:Cy5标记的羊抗小鼠IgG
英文名称:Goat Anti-Mouse IgG/Cy5
货号:ym-c0296G-Cy5
规格:0.1ml
级别:分子生物学级, 亲和纯化
抗原:多肽抗原
抗原来源:多肽合成
抗体来源:Rabbit
适用物种:hu, mo, rat
可提供抗体IgG的各种标记conjugate:可提供抗体IgG的各种标记 Isotype:可咨询
应用范围:WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
克隆类型:Polyclonalormonoclonal
交叉反应:Human,Mouse,Rat,Dog,Horse
保存环境:2-8℃ 短期保存或<-20℃长期保存保存1年以上,避免反复冻融。
Cy5标记的羊抗小鼠IgGRules:
(1) the initial reaction to produce antibodies to antigens: when the first to enter the body, need to produce antibodies after certain incubation period can, and antibody production quantity are not many, in the body to maintain the time is short.
(2) once again: when the same antigen antibody reaction to produce second times into the body, at the beginning, as part of the original antibody binding and once again into the antigen antibody, can make the original amount reduced slightly. Subsequently, rapid increase antibody titer, several times more than the initial reaction can be generated to a few times, retained in the body of a lot of time.
(3) recall response to produce antibodies: antibodies produced by antigen stimulation of the body, after a certain period of time can disappear gradually. If the re exposure to antigen, the antibody has faded fast rise. As the body with the same antigen again first, then known as the specific recall responses; and if the initial reactions of different, it is known as a nonspecific recall response. Increased nonspecific memories to elicit antibody responses are temporary, short time soon fall.
Cy5标记的羊抗小鼠IgG相关产品:
Mouse Anti-human IgG/PE-Cy7PE-Cy7标记的小鼠抗人IgGym-c0297M-PE-Cy7
Mouse Anti-human IgG/FITCFITC标记的小鼠抗人IgGym-c0297M-FITC
Mouse Anti-human IgG/PE-Cy5.5PE-Cy5.5标记的小鼠抗人IgGym-c0297M-PE-Cy5.5
Mouse Anti-human IgG/AP碱性磷酸酶(AP)标记的小鼠抗人IgGym-c0297M-AP
Goat Anti-human IgM/HRP辣根过氧化物酶标记的羊抗人IgMym-c0345G-HRP
Goat Anti-human IgM/Cy3Cy3标记的羊抗人IgMym-c0345G-Cy3
Goat Anti-human IgM/PE-CY5PE-CY5标记的羊抗人IgMym-c0345G-PE-CY5
Goat Anti-human IgM/PEPE标记的羊抗人IgMym-c0345G-PE
Goat Anti-human IgG/PE-Cy7PE-Cy7标记的羊抗人IgGym-c0297G-PE-Cy7
Goat Anti-Chicken IgG/Cy7Cy7标记的羊抗鸡IgGym-c0310G-Cy7
Goat Anti-Chicken IgG/PE-Cy7PE-Cy7标记的羊抗鸡IgGym-c0310G-PE-Cy7
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文献和实验,但是Dylight的效价通常是常规荧光二抗的2-4倍左右,因此就最终的性价比,Dylight更胜一筹。举例来说,EarthOx的FITC标记山羊抗小鼠IgG二抗 (货号为E031210 ),100μl和500μl报价分别是¥160和¥640;而相应的EarthOx的Dylight 488标记的山羊抗小鼠IgG二抗 (货号为E032210 ),100μl和500μl报价分别是¥200和¥800,但是在具体使用时,比如免疫荧光实验,其推荐稀释比率分别为1:100和1:400,即100μl和500μl的FITC标记山羊抗小鼠IgG
【共享】荧光二抗的选择——Dylight荧光与传统荧光标记的比较
比,Dylight更胜一筹。举例来说,EarthOx的FITC标记的山羊抗小鼠IgG二抗(货号为E031210),100μl和500μl报价分别是¥160和¥640;而相应的EarthOx的Dylight 488标记的山羊抗小鼠IgG二抗(货号为E032210),100μl和500μl报价分别是¥200和¥800,但是在具体使用时,比如免疫荧光实验,其推荐稀释比率分别为1:100和1:400,即100μl和500μl的FITC标记山羊抗小鼠IgG二抗(货号为E031210)最终使用液是10ml和50
可以将干扰扣除,但补偿值越大,补偿后的信号分辨率会降低越厉害。最好选择很少或者没有重叠的荧光基团,以最大限度减少这种渗漏。不同激光激发的染料是首选,如PE和APC,但在某些情况下,这可能与尽量选择亮的荧光素的规则是冲突的。如在进行四色分析时,经常会将PE-Cy5标记的抗体与APC标记的抗体搭配使用。因为Cy5的激发波长与APC的激发波长相近,所以PE-Cy5与APC均可被红色激光(640nm)所激发,而两者的发送光谱也比较接近,导致PE-Cy5与APC的颜色补偿很难调整。相反,由于PE-Cy5.5中的
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