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文献和实验. Assisted large fragment insertion with Red/ET-recombination (ALFIRE) ALFIRE is based on the rationale that HAs can be easily inserted directly into the accepting vector at the same time when the integration of an insert/selection
Miniprep Kit Procedure This figure shows the general In-Fusion BioBrick assembly scheme for BioBricks on pSB1A2 plasmids. See details below. Part A (blue) and Part B (red) are on pSB1A2 plasmids encoding ampicillin resistance. Primers described
Recombination-Mediated Genetic Engineering of Plasmodium berghei DNA
and to generate complex vectors for modifying the parasite genome. We here describe a protocol for using lambda Red recombinase to modify inserts of a P. berghei genomic DNA library constructed in a linear, low-copy, phage-derived vector. The method uses primer
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