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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4度,-20
- 保质期:
2年
- 英文名:
thermo
- 库存:
1000
- 供应商:
北诺生命科学
- 规格:
100 MG
货号 产品名称 规格 2013 目录价(¥)
K0331 Luminaris Color Probe High ROX qPCR Master Mix 250 x 20 µL rxns 1,355
K0332 Luminaris Color Probe High ROX qPCR Master Mix 500 x 20 µL rxns 2,510
K0333 Luminaris Color Probe High ROX qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0334 Luminaris Color Probe High ROX qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0341 Luminaris Color Probe Low ROX qPCR Master Mix 250 x 20 µL rxns 1,355
K0342 Luminaris Color Probe Low ROX qPCR Master Mix 500 x 20 µL rxns 2,510
K0343 Luminaris Color Probe Low ROX qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0344 Luminaris Color Probe Low ROX qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0351 Luminaris Color Probe qPCR Master Mix 250 x 20 µL rxns 1,355
K0352 Luminaris Color Probe qPCR Master Mix 500 x 20 µL rxns 2,510
K0353 Luminaris Color Probe qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0354 Luminaris Color Probe qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0361 Luminaris Color HiGreen High ROX qPCR Master Mix 250 x 20 µL rxns 1,355
K0362 Luminaris Color HiGreen High ROX qPCR Master Mix 500 x 20 µL rxns 2,510
K0363 Luminaris Color HiGreen High ROX qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0364 Luminaris Color HiGreen High ROX qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0371 Luminaris Color HiGreen Low ROX qPCR Master Mix 250 x 20 µL rxns 1,355
K0372 Luminaris Color HiGreen Low ROX qPCR Master Mix 500 x 20 µL rxns 2,510
K0373 Luminaris Color HiGreen Low ROX qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0374 Luminaris Color HiGreen Low ROX qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0381 Luminaris Color HiGreen Fluorescein qPCR Master Mix 250 x 20 µL rxns 1,355
K0382 Luminaris Color HiGreen Fluorescein qPCR Master Mix 500 x 20 µL rxns 2,510
K0383 Luminaris Color HiGreen Fluorescein qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0384 Luminaris Color HiGreen Fluorescein qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0391 Luminaris Color HiGreen qPCR Master Mix 250 x 20 µL rxns 1,355
K0392 Luminaris Color HiGreen qPCR Master Mix 500 x 20 µL rxns 2,510
K0393 Luminaris Color HiGreen qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0394 Luminaris Color HiGreen qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0441 TranscriptAid™ T7 High Yield Transcription Kit 50 react 2,325
K0512 Genomic DNA Purification Kit 100 preps 595
K0513 Silica Bead DNA Gel Extraction Kit 100 preps 541
K0621 DecaLabel™ DNA Labeling Kit 10 react 541
K0622 DecaLabel™ DNA Labeling Kit 30 react 1,352
K0651 Biotin DecaLabel™ DNA Labeling Kit 10 react 919
K0652 Biotin DecaLabel™ DNA Labeling Kit 30 react 2,271
K0661 Biotin Chromogenic Detection Kit 10 react 865
K0662 Biotin Chromogenic Detection Kit 30 react 1,947
K1071 DreamTaq™ PCR Master Mix (2X) 200 react 541
K1072 DreamTaq™ PCR Master Mix (2X) 1000 react 2,163
K1081 DreamTaq™ Green PCR Master Mix (2X) 200 react 541
K1082 DreamTaq™ Green PCR Master Mix (2X) 1000 react 2,163
K1091 Fast Tissue-to-PCR Kit 200 react 2,271
K1213 InsTAclone™ PCR Cloning Kit 10 react 649
K1214 InsTAclone™ PCR Cloning Kit 30 react 1,406
K1231 CloneJET™ PCR Cloning Kit 20 react 1,146
K1232 CloneJET™ PCR Cloning Kit 40 react 2,163
K1422 Rapid DNA Ligation Kit 50 react 1,179
K1423 Rapid DNA Ligation Kit 150 react 2,358
K1611 First Strand cDNA Synthesis Kit 20 react 595
K1612 First Strand cDNA Synthesis Kit 100 react 2,379
K1621 RevertAid™ First Strand cDNA Synthesis Kit 20 react 433
K1622 RevertAid™ First Strand cDNA Synthesis Kit 100 react 1,730
K1631 RevertAid™ H Minus First Strand cDNA Synthesis Kit 20 react 757
K1632 RevertAid™ H Minus First Strand cDNA Synthesis Kit 100 react 3,028
K1641 Maxima® First Strand cDNA Synthesis Kit 50 react 2,704
K1642 Maxima® First Strand cDNA Synthesis Kit 200 react 8,111
K1651 RevertAid™ Premium First Strand cDNA Synthesis Kit 20 react 1,049
K1652 RevertAid™ Premium First Strand cDNA Synthesis Kit 100 react 4,196
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文献和实验Phycoerythrin conjugation protocol
DMSO Dimethyl sulphoxide, anhydrous SPDP 3-(2-pyridyldithio)propionic acid N-hydroxysuccinimide ester (Sigma) SMCC Succinimidyl trans-4-(N-maleimidylmethyl)cyclohexane-1-carboxylate
PriCells: 藻红蛋白标记抗体的方法﹠PE-标记蛋白A方法
藻红蛋白标记抗体的方法:1. 巯基化藻红蛋白(PE)的制备;(1)将600μl浓度为15.5mg/ml的盐酸巯醇亚胺加到1.2ml浓度为3.6mg/ml的PE中;(2)将以上混合液和1.2ml pH6.8的PB混合,而后装入透析袋置入50mmol/L pH6.8的PB中于4℃透析,过夜;(3)再换用pH7.5 PB透析6h。每个PE分子中可结合8个巯基;2. 巯基PE-IgG的制备;(1)将30μl浓度为1.1mg/ml的异双功能试剂SPDP的乙醇溶液,加入到700μl
巯基化探针引入到二硫化膜介质上,即可发生巯基-交换反应,然后探针通过二硫键连接到膜上。巯基化探针与处理膜表面的连接反应证明上述反应。巯基-交换反应快速并可定量,唯一弊端是巯基化探针使用前可能形成引物二聚体。并且通过阻隔二硫键形成引物二聚体大的试剂都会干扰免疫探针二硫键形成。许多能用来交联的化学试剂可应用于巯基和胺基异源双功能连接。通常情况下,应用到巯基化核苷连接的常用试剂有NHS、MBS及SPDP.前面提到的巯基吡哚连接中二硫键的大多是SPDP催化形成,然而,二硫键易形成链内连接而不是在探针和链
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