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- CST
- USA
- 9379S
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
2-10年
- 英文名：
Cell Signaling Technology
- 库存：
1000
- 供应商：
CST
- CAS号：
Polyclonal Antibody
- 规格：
100 ul
美国 Cell Signaling Technology（CST）是最知名的和老牌的信号转导公司之一，专注于信号转导产品的提供和研究。产品具有种类多、质量好、价格低、引用文献多等优点，是您进行信号转导研究不可多得的帮手！
提供特色的信号传导用磷酸化检测抗体、ELISA检测试剂盒、活性的重组激酶等药筛产品。涉及的细胞信号通路有： 1 染色质调控与乙酰化信号通路 2 MAP激酶信号网络 3 SAPK/JNK信号通路 4 细胞凋亡与Caspase信号网络 5 AKT信号网络 6 转录与翻译调控 7 蛋白激酶C PKC 信号网络 8 周期调控与DNA损伤信号通路 9 细胞骨架调控 10 白细胞信号传导，等等。
主要产品：
抗体类：提供超过1300种磷酸化和非磷酸化抗体，具有最多的磷酸化抗体，同时提供sample kit和phosphoPlus Ab kit
ELISA试剂盒：PathScan Sandwich ELISA Kit系列产品是检测蛋白磷酸化水平的ELISA试剂盒，灵敏度高，操作简单
WB用蛋白Marker：CST的Biotinylated Protein Ladder Detection Pack产品包括有10个条带、大小在10-200Kda之间的生物素标记蛋白标准和HRP藕联的抗生物素二抗（anti-biotin antibody, HRP-linked），有大小两种包装可选
kinase assay kit：试剂盒组成合理全面，非同位素操作，应用起来简单方便。
buffer类：共有6个buffer类，是进行普通WB或磷酸化WB检测的必须辅助试剂，用于标本蛋白的制备。
激酶类：多种形式的蛋白激酶，可以用于药物筛选
药物筛选试剂盒：高通量、针对激酶的药物筛选
The genomic era has yielded a wealth of new genes for which function and inter-relationships remain unknown. At the same time there is an increasing appreciation of the limits of what can be learned from gene expression profiling, and of the need to examine protein expression and activity. Activation-state-specific antibodies have proven to be a convenient and reliable set of tools in the new post-genomic "research toolbox". Cell Signaling Technology, Inc. （CST） is committed to developing innovative new research tools such as these to help define the mechanisms underlying cell function and disease, thereby broadly accelerating progress in biomedical research and medicine.
CST strives to make novel antibodies of the highest possible quality by producing them in-house and rigorously characterizing them with respect to specificity and sensitivity. Production begins with extensive purification （typically by both protein A and peptide affinity chromatography） followed by testing by our staff scientists on a wide range of applications, including ELISA, western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry and flow cytometry. Technical support is provided by the very scientists who make a given antibody and know it best. CST scientists are also responsible for creating most of the cell signaling reference material, including pathways, kinase trees and kinase disease associations that we offer our customers in the last chapter of this catalog and on our website.
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文献和实验提示我模型中的通路究竟涉及哪个亚型，而我又没有那么多经费一个一个亚型去试，故我想能不能就检测总的PKC激活水平，毕竟有亚型被激活，那总的活化PKC水平也应该会升高啊！虽然敏感性和特异性肯定没有单独检测一个亚型的高。 还想请教：Phospho-PKC (pan) (gamma Thr514) Antibody 和Phospho-PKC (pan) (βII Ser660) Antibody 这两个抗体的针对的亚型有什么不同？既然都是pan，那是否说都是针对所有的亚型，只是磷酸化的残基不同
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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