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- 2025年07月02日
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文献和实验Southern Blot using Neutral Transfer
is better for resolving larger fragments. Add ethidium to buffer near red ( ). (0.1 μg/ml ethidium should be included in the gel for visualization). 3) Run gel 5-6 hours at 40 to 60 volts. Once gel is finished running, be sure to take a picture with ruler
Standard neutral agarose electrophoresis
Standard neutral agarose electrophoresis Standard agarose gels can be prepared using either TBE or TAE running buffers. You will need: Either 10 x TBE or 10 x TAE buffers 1 x TBE buffer - 89mM Tris, 89mM boric acid, 2mM EDTA, pH
Array CGH of Microdissected Fresh or Formalin Fixed DNA
is cleaned up to remove unincorporated nucleotides and for buffer exchange into water for the next step of random prime labeling. ・ Add 125 ml of PB buffer to RPA reaction (5x volume) ・ Place Qiaquick column in 2 ml tube, pipet reaction
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