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- 2025年08月05日
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文献和实验a funnel in the front porthole of the formalin mixing tank and pour sodium chloride solution into the main tank. Replace screw cap on porthole. Using the sealed pump attached to the formalin mixing tank , add 10 litres of formaldehyde to the main
the sealed pump attached to the formalin mixing tank, pump 50 litres into the top tank where the solution is ready for use. 10% formal-saline solution can be dispensed from the tap with the red knob on the cut-up bench. NB
Southern Blot using Neutral Transfer
is better for resolving larger fragments. Add ethidium to buffer near red ( ). (0.1 μg/ml ethidium should be included in the gel for visualization). 3) Run gel 5-6 hours at 40 to 60 volts. Once gel is finished running, be sure to take a picture with ruler
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