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群晓科苑
BlotGlyco® 聚糖纯化和标记试剂盒
BlotGlyco® Glycan purification and labeling kit

试剂盒成分如下:
| 货号 |
产品名称 |
试剂盒组分 |
||||
| Polymer beads |
Labeling reagent for MALDI-TOF MS (aoWR) |
Reaction tube |
Cleanup column |
Sample tube (2mL) |
||
| BS-45603Z |
BlotGlyco®10 |
10 assays (50mg) |
10 assays |
10 |
10 |
10 |
| BS-45604Z |
BlotGlyco®50 |
50 assays (250mg) |
50 assays |
50 |
50 |
50 |
| BS-45605Z |
BlotGlyco®100 |
100assays (500mg) |
100 assays |
100 |
100 |
100 |
| BS-45606Z |
BlotGlyco®100A |
100assays (500mg) |
100 assays |
- |
- |
- |
| BS-45607Z |
BlotGlyco®100B |
100assays (500mg) |
- |
100 |
100 |
100 |
| BS-45608Z |
BlotGlyco®100C |
100assays (500mg) |
- |
- |
- |
- |
| BS-45609Z |
BlotGlyco®100D |
100assays (500mg) |
- |
- |
100 |
- |
| BS-45610Z |
BlotGlyco®200C |
200 assays (1000mg) |
- |
- |
- |
- |
| BS-45611Z |
BlotGlyco®100E |
100 assays (500mg) |
- |
100 |
- |
- |
试剂盒组分展示如下:

BlotGlyco® beads (freeze-dried) aoWR :Labeling reagent for MALDI-TOF MS

Reaction tube Cleanup column Sample tube (2mL)
实验原理:
BlotGlyco® beads含有高密度的酰肼基团:


第一步:聚糖纯化:beads上的酰肼基团与样品中的聚糖共价结合,达到纯化聚糖,去除蛋白、DNA等杂质的作用;
第二步:聚糖标记与分析:捕获的聚糖进而(经相应试剂标记)用于MALDI-TOF-MS或HPLC分析。
A. MALDI-TOF-MS分析:标签aoWR(试剂盒中含有的独特的标记试剂)通过-ONH2与聚糖结合,从而使其从beads上脱离下来,再用MALDI-TOF MS(基质辅助激光解吸电离飞行时间质谱)检测聚糖的类型与含量,灵敏度高。

B. HPLC (或LC-MS)分析:温和酸性条件下洗脱聚糖,用荧光标记试剂,如2AB (2-aminobenzamide),PA (2-aminopyridine),2AA (2-aminobenzoic acid)等结合洗脱下的聚糖,再用HPLC、LC-MS检测聚糖的类型与含量。

如果样品多时可以选择BlotGlyco® 96孔板进行检测
BlotGlyco® 96孔板聚糖检测
Glycan purification and labeling kit BlotGlyco® 96 well plate
BlotGlyco® 96孔板聚糖检测优点:
- 适用于高通量检测,操作简单
- 可用2AB等荧光标记检测
- 可用HPLC、LC-MS、MALDI-TOF MS等快速检测方法进行检测
- 适用于自动化进样系统
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文献和实验ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)
analysis of genomic DNA by southern blotting
, agarose gel electrophoresis, gel extraction of appropriate band. Resuspend in 10 mM Tris. Label 25-50 ng cDNA-probe with 32 P using the Prime It® II Random Primer Labeling Kit (Stratagene #300385). Supplies & Equipment:
Isolate It All:siRNA • miRNA • Total RNA • Native Protein
or by solution hybridization assay with the mir Vana™ miRNA Detection Kit (Ambion). RNA probes were prepared by in vitro transcription (mRNA probes--MAXIscript® Kit, Ambion) or by 5' end labeling (rRNA, miRNA and siRNA probes - mir Vana™ Probe & Marker Kit
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